|Description||Goat Anti-Rabbit IgG (H&L) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb. Anti Rabbit Conjugated Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other anibody detection methods.Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins|
|Conjugation||Alkaline Phosphatase (Calf Intestine)|
|Immunogen||Rabbit IgG whole molecule|
|Concentration||Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Specificity||Rabbit IgG (H&L) Alkaline Phosphatase Pre-Adsorbed Conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.|
|Storage||Store secondary antibody at 4° C before opening. DO NOT FREEZE. Alkaline Phosphatase conjugated antibodies are stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
|ELISA: 1:10,000 - 1:50,000
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:500 - 1:2,500
Other Dilution: User Optimized
Western Blot of Goat anti-Rabbit IgG Pre-Adsorbed Alkaline Phosphatase Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 100 ng per lane.Primary antibody: None.Secondary antibody: Alkaline Phosphatase goat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
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