|Description||Goat Anti-Rabbit IgG (H&L) antibody Biotin conjugated, pAb. Biotin Conjugated Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other anibody detection methods.Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins|
|Immunogen||Rabbit IgG whole molecule|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.|
|Storage||Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|ELISA: 1:20,000 - 1:400,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized
Western blot of Biotin conjugated Goat Anti-Rabbit IgG Pre-Adsorbed secondary antibody.Lane 1: Rabbit IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: Biotin conjugated Goat Anti-Rabbit IgG Pre-Adsorbed at 1:1,000 for 60 min at RT.Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG.Other band(s): None.
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