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Catalog Products » Other Products » Goat Anti-Rabbit IgG (H&L),pAb

Goat Anti-Rabbit IgG (H&L),pAb

Description Goat Anti-Rabbit IgG (H&L) antibody Unconjugated, pAb. Goat Anti-Rabbit IgG (H&L) antibody Unconjugated, pAb.Min X Human Serum Proteins
Host Species Goat
Antigen Species Rabbit
Conjugation Unconjugated
Immunogen Rabbit IgG whole molecule

Subclass IgG
Concentration Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Human Serum Proteins.  Specificity was confirmed by ELISA at less than 1%.
Storage Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

ELISA: 1:35,000 - 1:325,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:3,000 - 1:35,000
Other Dilution: User Optimized

  • Goat Anti-Rabbit IgG (H&L),pAb
  • Goat Anti-Rabbit IgG (H&L),pAb

    Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Cat. No. A10163). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Cat. No. A10163) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.




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Cat. No. A10165
Size
Price
2 mg $104.00