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Western Blot of Goat anti-Rabbit Fc antibody. Lane 1: Rabbit Fc. Lane 2: None. Load: 100 ng per lane.Primary antibody: Rabbit Fc antibody at 1:1,000 for overnight at 4°C.Secondary antibody: DyLight™ 649 goat secondary antibody at 1:20,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 28 kDa, 28 kDa for Rabbit Fc. Other band(s): Rabbit Fc splice variants and isoforms.
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