|Description||Goat Anti-Rabbit IgG F(c) antibody Unconjugated, pAb. Anti-Rabbit IgG F(c) antibody generated in goat detects specifically rabbit IgG F(c). This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.Min X Human Serum Proteins|
|Immunogen||Anti Rabbit IgG F(c) was produced by repeated immunization with rabbit IgG F(c) fragment in goat.|
|Concentration||Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab) or Human Serum Proteins.|
|Storage||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|ELISA: 1:20,000 - 1:100,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized
Western Blot of Goat anti-Rabbit Fc antibody. Lane 1: Rabbit Fc. Lane 2: None. Load: 100 ng per lane.Primary antibody: Rabbit Fc antibody at 1:1,000 for overnight at 4°C.Secondary antibody: DyLight™ 649 goat secondary antibody at 1:20,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 28 kDa, 28 kDa for Rabbit Fc. Other band(s): Rabbit Fc splice variants and isoforms.
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