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Catalog Products » Other Products » Goat Anti-Rabbit IgG (H&L) [FITC],pAb

Goat Anti-Rabbit IgG (H&L) [FITC],pAb

Description This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Host Species Goat
Antigen Species Rabbit
Conjugation Fluorescein (FITC)
Immunogen Rabbit IgG whole molecule

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Species Reactivity Rabbit
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.


FLISA: 1:10,000 - 1:50,000
Flow Cytometry: 1:500 - 1:2,500
IF Microscopy: 1:1,000 - 1:5,000
Other Dilution: User Optimized

Goat Anti-Rabbit IgG (H&L) [FITC],pAb
Goat Anti-Rabbit IgG (H&L) [FITC],pAb

Rockland FITC (fluorescein) and HRP (horse radish peroxidase) conjugated secondary antibody was used to detect nanogram – picogram levels of rabbit IgG by dot blot on nitrocellulose membrane. 4 ul each of serial 1 in 4 dilutions of rabbit IgG were dotted on nitrocellulose and allowed to dry. Membrane was blcoked in 3% BSA for 10 minutes dried for later use and rewetted with Blocking Buffer for Fluorescent Western Blotting. Blot was incubated in Rockland fluorescein conjugated goat anti rabbit 611-1202 lot 25176 1:10,000 and Rockland HRP conjugated goat anti Rabbit (611-1302 lot 25406 1:10,000, dried and:A. Blot was imaged on the BioRad VersaDoc with filter settings appropriate for Fluorescein/DyLight 488B. Blot was rewetted with TBS, incubated with FEMTOMAX chemiluminescent substrate for 1-3 minutes and imaged for 60sec on the BioRad VersaDoc Imaging SystemC. Blot was rinsed with TBS and DIH2O, incubated for 5 minutes with Rockland TMB Substrate for Western Blot MaxTag (1 ml of TMBM-102 + ~9 ml of TMBM-101), dried overnight and imaged using a conventional flatbed scanner




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Cat. No. A10167
Size
Price
2 mg $101.00
24 hour
 
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