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Catalog Products » Other Products » Goat Anti-Rabbit IgG (H&L) [HRP],pAb

Goat Anti-Rabbit IgG (H&L) [HRP],pAb

Description Rabbit secondary antibody conjugated to horseradish peroxidase (HRP) is available in a variety of formats. HRP Anti IgG secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western Blotting as well as other HRP antibody based assays.
Host Species Goat
Antigen Species Rabbit
Conjugation Peroxidase (Horseradish)
Immunogen Rabbit IgG whole molecule

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Storage Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:150,000 - 1:250,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:10,000 - 1:20,000
Other Dilution: User Optimized

Goat Anti-Rabbit IgG (H&L) [HRP],pAb
Goat Anti-Rabbit IgG (H&L) [HRP],pAb

Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Cat. No. A10163). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Cat. No. A10163) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.




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Cat. No. A10169
Size
Price
2 mg $120.00
24 hour
 
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