|Description||Goat Anti-Rabbit IgG F(c) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Rabbit IgG Fc antibody generated in goat detects specifically Rabbit IgG Fc fragments. This anti-Rabbit secondary antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Rabbit IgG F(c) fragment was produced by repeated immunization with Rabbit IgG F(c) fragment in goat.|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||Anti-Rabbit IgG F(c) antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab)|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:20,000
Other Dilution: User Optimized
Western blot of Peroxidase conjugated Goat Anti-Rabbit IgG F(c) secondary antibody.Lane 1: Rabbit IgG F(c).Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 28 kDa, 28 kDa for Rabbit IgG F(c).Other band(s): None.
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