|Description||Goat Anti-Rabbit IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Rabbit IgG peroxidase conjugated antibody generated in goat detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.Min X Human Serum Proteins|
|Immunogen||Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|ELISA: 1:50,000 - 1:100,000
Immunohistochemistry: 1:750 - 1:2,500
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized
Western Blot of Goat anti-Rabbit IgG Peroxidase Conjugated Antibody. Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH 3T3 Whole Cell Lysate. Load: 10 µg per lane.Primary antibody: Beta Actin antibody at 1:2,000 for overnight at 4°C.Secondary antibody (2 Blots on Left): Atto 647N goat secondary antibody at 1:10,000 for 60 min at RT.Secondary Antibody (2 Blots on Right): Peroxidase goat secondary antibody at 1:10,000 for 60 min at RT.Block: 5% BLOTTO overnight at 4°C.Predicted/Observed size: 50 kDa, 50 kDa for Beta Actin. Other band(s): none.
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