|Description||Goat Anti-Rabbit IgG (H&L) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb. Anti-Rabbit IgG alkaline phosphatase conjugated antibody generated in goat detects specifically rabbit IgG. This secondary alkaline phosphatase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Conjugation||Alkaline Phosphatase (Calf Intestine)|
|Immunogen||Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.|
|Concentration||Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Specificity||RABBIT IgG (H&L) Antibody Alkaline Phosphatase conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG and Rabbit Serum.|
|Storage||Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:1,000 - 1:4,000
Other Dilution: User Optimized
Western Blot of Goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated.Lane 1: Rabbit IgG. Lane 2: None.Load: 100 ng per lane.Primary Antibody: None.Secondary antibody: Alkaline Phosphatase goat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 and 28 kDa, 55 kDa for Rabbit IgG.Other Band(s): None.
For more documents, please visit "Technical Support".