|Description||Goat Anti-Rabbit IgG F(c) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb. Goat Anti-Rabbit IgG F(c) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb.Min X Human Serum Proteins|
|Conjugation||Alkaline Phosphatase (Calf Intestine)|
|Immunogen||Rabbit IgG F(c) fragment|
|Concentration||Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab)or Human Serum Proteins.|
|Storage||Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
|ELISA: 1:2,000 - 1:10,000
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:500 - 1:2,500
Other Dilution: User Optimized
Dot Blot of Goat anti-Rabbit IgG Fc Antibody (Min X Human) Alkaline Phosphatase Conjugated. Antigen: Rabbit IgG Fc. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.Primary antibody: none.Secondary antibody: Goat anti-Rabbit IgG Fc Antibody (Min X Human) Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT.Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.
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