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Goat Anti-Rabbit IgG (H&L) [BAC],pAb

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Overview

Description	Goat Anti-Rabbit IgG (H&L) antibody Biotin conjugated, pAb. Anti-Rabbit IgG whole molecule antibody generated in goat detects specifically Rabbit IgG whole molecule. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species	Goat
Antigen Species	Rabbit
Conjugation	Biotin
Immunogen	Anti-Rabbit IgG whole molecule was produced by repeated immunization with Rabbit IgG whole molecule in goat.

Properties

Subclass	IgG
Concentration	Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution	Restore with deionized water (or equivalent)
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Species Reactivity	Rabbit
Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Applications

ELISA: 1:50,000 - 1:200,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:5,000 - 1:20,000
Other Dilution: User Optimized

Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Cat. No. A10163). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Cat. No. A10163) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.