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Catalog Products » Other Products » Sheep Anti-Rabbit IgG (H&L) [HRP],pAb

Sheep Anti-Rabbit IgG (H&L) [HRP],pAb

Description Sheep Anti-Rabbit IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Rabbit IgG peroxidase conjugated antibody generated in sheep detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Sheep
Antigen Species Rabbit
Conjugation Peroxidase (Horseradish)
Immunogen Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in sheep.

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:10,000 - 1:50,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized

  • Sheep Anti-Rabbit IgG (H&L) [HRP],pAb
  • Sheep Anti-Rabbit IgG (H&L) [HRP],pAb

    Western blot of Peroxidase Conjugated Sheep Anti-Rabbit IgG secondary antibody.Lane 1: Rabbit IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Peroxidase sheep secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG.Other band(s): None.




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Cat. No. A10185
Size
Price
2 mg $129.00
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