|Description||Sheep Anti-Rabbit IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Rabbit IgG peroxidase conjugated antibody generated in sheep detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in sheep.|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Rabbit IgG and Rabbit Serum.|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|ELISA: 1:10,000 - 1:50,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Western blot of Peroxidase Conjugated Sheep Anti-Rabbit IgG secondary antibody.Lane 1: Rabbit IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Peroxidase sheep secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG.Other band(s): None.
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