|Description||Donkey Anti-Rabbit IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins|
|Immunogen||Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in donkey.|
|cannot ship to canada||cannotShipToCanada|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|ELISA: 1:10,000 - 1:100,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase donkey secondary antibody at 1:1,000 for 60 min at RTBlock: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none.
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