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Home » Gene Synthesis & Molecular Biology Services » Donkey Anti-Rabbit IgG (H&L) [ALP],pAb

Donkey Anti-Rabbit IgG (H&L) [ALP],pAb

*This product has been discontinued!*
Description Donkey Anti-Rabbit IgG (H&L) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb. Anti-Rabbit IgG (H&L) antibody generated in donkey detects specifically rabbit IgG (H&L). This secondary antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins
Host Species Donkey
Antigen Species Rabbit
Conjugation Alkaline Phosphatase (Calf Intestine)
Immunogen Anti-Rabbit IgG (H&L) was produced by repeated immunization with Rabbit IgG whole molecule in donkey

Subclass IgG
Concentration Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Species Reactivity Rabbit
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

ELISA: 1:10,000 - 1:20,000
Immunohistochemistry: 1:400 - 1:2,000
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized




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