|Description||Donkey Anti-Rabbit IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in donkey.|
|cannot ship to canada||cannotShipToCanada|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
Immunohistochemistry: 1:500 - 1:2,000
Western Blot: 1:1,000 - 1:3,000
Other Dilution: User Optimized
Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase donkey secondary antibody at 1:1,000 for 60 min at RTBlock: Blocking Buffer for Fluorescent Western Blotting for 30 min RT.Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none.
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