Catalog Products » Other Products » Donkey Anti-Rabbit IgG (H&L) [BAC],pAb

Donkey Anti-Rabbit IgG (H&L) [BAC],pAb

Description Donkey Anti-Rabbit IgG (H&L) antibody Biotin conjugated, pAb.
Host Species Donkey
Antigen Species Rabbit
Conjugation Biotin
Immunogen Rabbit IgG whole molecule
cannot ship to canada cannotShipToCanada

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:300,000
Immunohistochemistry: 1:500 - 1:3000
Western Blot: 1:3,000 - 1:15,000
Other Dilution: User Optimized

  • Donkey Anti-Rabbit IgG (H&L) [BAC],pAb
  • Donkey Anti-Rabbit IgG (H&L) [BAC],pAb

    Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated (Cat. No. A10198). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated (Cat. No. A10198) 1:5,000 for 60 min at RT. Secondary antibody: None. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

Notice there is no results found, you can visit "Citations Database" for other results.

For more documents, please visit "Technical Support".

Cat. No. A10200
2 mg $114.00