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Catalog Products » Other Products » Donkey Anti-Rabbit IgG (H&L) [TR],pAb

Donkey Anti-Rabbit IgG (H&L) [TR],pAb

Description Donkey Anti-Rabbit IgG (H&L) antibody Texas Red® conjugated, pAb. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Host Species Donkey
Antigen Species Rabbit
Conjugation Texas Red®
Immunogen Rabbit IgG whole molecule

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG, and Rabbit Serum.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.


FLISA: 1:10,000 - 1:50,000
Flow Cytometry: 1:500 - 1:2,500
IF Microscopy: 1:1,000 - 1:5,000
Other Dilution: User Optimized

  • Donkey Anti-Rabbit IgG (H&L) [TR],pAb
  • Donkey Anti-Rabbit IgG (H&L) [TR],pAb

    Dot Blot showing the detection of Rabbit IgG. A three-fold serial dilution of Rabbit IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto 1 Hour at 20°C, Anti-Rabbit IgG (H&L) (DONKEY) Antibody Texas Red Conjugated (Cat. No. A10201) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting and imaged using the Bio-Rad VersaDoc® 4000 MP.




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Cat. No. A10201
Size
Price
2 mg $114.00
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