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Catalog Products » Other Products » Goat Anti-Rat IgG (H&L),pAb

Goat Anti-Rat IgG (H&L),pAb

Description Goat Anti-Rat IgG (H&L) antibody Unconjugated, pAb. RAT IgG (H&L) (GOAT) Antibody was generated in goat detects specifically Rat IgG whole molecule. Anti-Rat IgG antibosy is ideal for investigators involved in serum protein component research.Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins
Host Species Goat
Antigen Species Rat
Conjugation Unconjugated
Immunogen Rat IgG whole molecule

Subclass IgG
Concentration Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Specificity Anti-RAT IgG (H&L) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Species Reactivity Rat
Storage Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

ELISA: 1:10,000 - 1:40,000
Immunohistochemistry: 1:500 - 1:2,000
Western Blot: 1:1,000 - 1:4,000
Other Dilution: User Optimized

  • Goat Anti-Rat IgG (H&L),pAb
  • Goat Anti-Rat IgG (H&L),pAb

    Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (Cat. No. A10203). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (Cat. No. A10203) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.




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Cat. No. A10203
Size
Price
1 mg $123.00
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