|Description||Goat Anti-Rat IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Rat IgG whole molecule antibody generated in goat detects specifically Rat IgG whole molecule. This secondary antibody anti-Rat is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins|
|Immunogen||Anti-Rat IgG whole molecule was produced by repeated immunization with Rat IgG whole molecule in goat.|
|cannot ship to canada||cannotShipToCanada|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
Immunohistochemistry: 1:500 - 1:1,500
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized
Western Blot of Peroxidase conjugated Goat anti-Rat IgG antibody. Lane 1: Rat IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase rat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 kDa, 28 kDa for Rat IgG. Other band(s): none.
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