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Catalog Products » Other Products » Goat Anti-Rat IgG (H&L) [HRP],pAb

Goat Anti-Rat IgG (H&L) [HRP],pAb

Description Goat Anti-Rat IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Rat IgG whole molecule antibody generated in goat detects specifically Rat IgG whole molecule. This secondary antibody anti-Rat is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins
Host Species Goat
Antigen Species Rat
Conjugation Peroxidase (Horseradish)
Immunogen Anti-Rat IgG whole molecule was produced by repeated immunization with Rat IgG whole molecule in goat.
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Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Species Reactivity Rat
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:70,000
Immunohistochemistry: 1:500 - 1:1,500
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

  • Goat Anti-Rat IgG (H&L) [HRP],pAb
  • Goat Anti-Rat IgG (H&L) [HRP],pAb

    Western Blot of Peroxidase conjugated Goat anti-Rat IgG antibody. Lane 1: Rat IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase rat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 kDa, 28 kDa for Rat IgG. Other band(s): none.




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Cat. No. A10204
Size
Price
1 mg $195.00
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