SC2.0 More Details »
Click for More...
Western Blot of Anti-Rat IgM (mu chain) (GOAT) Antibody (Cat. No. A10208). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody (Cat. No. A10208) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
For more documents, please visit "Technical Support".
Quote for Customized Size
Please contact us if you need immediate assistance.
Please contact us (phone: 1-877-436-7274 or email: email@example.com),if you need immediate assistance.