|Description||Goat Anti-Rat IgG (H&L) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb.|
|Conjugation||Alkaline Phosphatase (Calf Intestine)|
|Immunogen||Rat IgG whole molecule|
|Concentration||Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgG and Rat Serum.|
|Storage||Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
|ELISA: 1:50,000 - 1:90,000
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:500 - 1:2,500
Other Dilution: User Optimized
Western Blot of Alkaline Phosphatase Conjugated Goat anti-Rat IgG antibody. Lane 1: Rat IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Alkaline Phosphatase rat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min RT.Predicted/Observed size: 55 kDa, 28 kDa for Rat IgG. Other band(s): none.
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