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Home » Gene Synthesis & Molecular Biology Services » Goat Anti-Rat IgG (H&L) [ALP],pAb

Goat Anti-Rat IgG (H&L) [ALP],pAb

Description Goat Anti-Rat IgG (H&L) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb.
Host Species Goat
Antigen Species Rat
Conjugation Alkaline Phosphatase (Calf Intestine)
Immunogen Rat IgG whole molecule

Subclass IgG
Concentration Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgG and Rat Serum.
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

ELISA: 1:50,000 - 1:90,000
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:500 - 1:2,500
Other Dilution: User Optimized

  • Goat Anti-Rat IgG (H&L) [ALP],pAb
  • Goat Anti-Rat IgG (H&L) [ALP],pAb

    Western Blot of Alkaline Phosphatase Conjugated Goat anti-Rat IgG antibody. Lane 1: Rat IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Alkaline Phosphatase rat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min RT.Predicted/Observed size: 55 kDa, 28 kDa for Rat IgG. Other band(s): none.

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Cat. No. A10215
1 mg $153.00