|Description||Goat Anti-Rat IgG (H&L) antibody Biotin conjugated, pAb. RAT IgG Biotin Conjugated antibody detects rat immunoglobulin G. Immunoglobuoin G is a molecule of about 150 kDa composed of four peptide chains. Each IgG contains two identical γ heavy chains of about 51 kDa and two identical light chains of about 26 kDa, thus a tetrameric quaternary structure. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape. Each end of the fork contains an identical antigen binding site. The Fc regions of IgGs bear a highly conserved N-glycosylation site.|
|Immunogen||Rat IgG whole molecule|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||Anti-RAT IgG Biotin Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgG and Rat Serum.|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|ELISA: 1:20,000 - 1:100,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized
Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated.Lane 1: Rat IgG.Load: 100 ng per lane.Primary antibody: Rat IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT.Secondary antibody: HRP Conjugated Streptavidin at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.
For more documents, please visit "Technical Support".