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Rabbit Anti-Rat IgG (H&L),pAb

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Overview

Description	Rabbit Anti-Rat IgG (H&L) antibody Unconjugated, pAb. Anti-Rat IgG (H&L) antibody generated in rabbit detects specifically rat IgG (H&L). This secondary antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.Min X Human Serum Proteins
Host Species	Rabbit
Antigen Species	Rat
Conjugation	Unconjugated
Immunogen	Anti-Rat IgG (H&L) was produced by repeated immunization with rat IgG whole molecule in rabbit

Properties

Subclass	IgG
Concentration	Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.
Species Reactivity	Rat
Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Applications

ELISA: 1:20,000 - 1:300,000
Immunohistochemistry: 1:1,000-1:5,000
Western Blot: 1:2,000-1:10,000
Other Dilution: User Optimized

Western Blot of Anti-Rat IgG (H&L) (RABBIT) Antibody (Min X Human Serum Proteins) (Cat. No. A10218). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (RABBIT) Antibody (Min X Human Serum Proteins) (Cat. No. A10218) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (Cat. No. A10160) 1:40,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.