×
Catalog Products » Other Products » Donkey Anti-Rat IgG (H&L),pAb

Donkey Anti-Rat IgG (H&L),pAb

Description Donkey Anti-Rat IgG (H&L) antibody Unconjugated, pAb.
Host Species Donkey
Antigen Species Rat
Conjugation Unconjugated
Immunogen Rat IgG whole molecule
cannot ship to canada cannotShipToCanada

Subclass IgG
Concentration Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rat IgG and Rat Serum.
Storage Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

ELISA: 1:20,000 - 1:100,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

  • Donkey Anti-Rat IgG (H&L),pAb
  • Donkey Anti-Rat IgG (H&L),pAb

    Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.




Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. A10224
Size
Price
2 mg $91.00
*
*
*
*