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Catalog Products » Other Products » Donkey Anti-Rat IgG (H&L) [HRP],pAb

Donkey Anti-Rat IgG (H&L) [HRP],pAb

Description Donkey Anti-Rat IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb.
Host Species Donkey
Antigen Species Rat
Conjugation Peroxidase (Horseradish)
Immunogen Rat IgG whole molecule
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Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rat IgG and Rat Serum.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:10,000 - 1:50,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:10,000
Other Dilution: User Optimized

  • Donkey Anti-Rat IgG (H&L) [HRP],pAb
  • Donkey Anti-Rat IgG (H&L) [HRP],pAb

    Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.




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Cat. No. A10225
Size
Price
2 mg $129.00
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