|Description||Goat Anti-Monkey IgA antibody Unconjugated, pAb. Anti-Monkey IgA (alpha chain) antibody generated in goat detects specifically monkey IgA (alpha chain). This secondary antibody anti-Monkey is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Monkey IgA (alpha chain) was produced by repeated immunization with monkey IgA alpha heavy chain in goat.|
|Concentration||Liquid (sterile filtered) , 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgA and Monkey Serum. No reaction was observed against Monkey IgG or Monkey IgM. Specificity was confirmed by ELISA at less than 1% cross reactivity against other monkey heavy or light chain isotypes.|
|Storage||Store vial at 4° C prior to opening.This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.For extended storage aliquot contents and freeze at -20° C or below.Avoid cycles of freezing and thawing.|
Other Dilution: User Optimized
Western Blot of Goat anti-Monkey IgA (alpha chain) antibody.Lane 1: Human IgA.Lane 2: None. Load: 100 ng per lane.Primary antibody: Monkey IgA (alpha chain) antibody at 1:1000 for overnight at 4°C.Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting 30 min at RT.Predicted/Observed size: 60 kDa, 60 kDa for Monkey IgA.Other Band(s): None.
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