|Description||Goat Anti-Monkey IgG (γ chain) antibody Unconjugated, pAb. Anti-Monkey IgG (gamma chain) antibody generated in goat detects specifically monkey IgG (gamma chain). This secondary antibody anti-Monkey is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Monkey IgG (gamma chain) was produced by repeated immunization with monkey IgG gamma heavy chain in goat.|
|Concentration||Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgG and Monkey Serum. No reaction was observed against Monkey IgM or Monkey IgA.|
|Storage||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|ELISA: 1:100,000 - 1:250,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized
Western Blot of Goat anti-Monkey IgG gamma Antibody.Lane 1: Monkey IgG.Lane 2: None.Load: 100 ng per lane.Primary antibody: Monkey IgG gamma Antibody at 1:1000 for overnight at 4°C.Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 kDa, 55 kDa for Monkey IgG Gamma. Other band(s): None.
For more documents, please visit "Technical Support".