|Description||Goat Anti-Monkey IgA antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Monkey IgA (alpha chain) peroxidase conjugated antibody generated in goat detects specifically Monkey IgA. This secondary peroxidase conjugated antibody anti-Monkey is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Monkey IgA (alpha chain) was produced by repeated immunization with monkey IgA alpha heavy chain in goat.|
|cannot ship to canada||cannotShipToCanada|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgA and Monkey Serum. No reaction was observed against Monkey IgM or Monkey IgG.|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4° C as an undiluted liquid.Dilute only prior to immediate use.|
|ELISA: 1:10,000 - 1:150,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:10,000
Other Dilution: User Optimized
Western Blot of Peroxidase conjugated Goat anti-MONKEY IgA alpha antibody. Lane 1: Human IgA. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 60 kDa for Monkey IgA. Other band(s): none.
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