|Description||Goat Anti-Monkey IgM µ chain antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Monkey IgM (mu chain) peroxidase conjugated antibody generated in goat detects specifically Monkey IgM (mu chain). This secondary peroxidase conjugated antibody anti-Monkey is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Monkey IgM (mu chain) was produced by repeated immunization with monkey IgM mu heavy chain in goat.|
|cannot ship to canada||cannotShipToCanada|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgM and Monkey Serum. No reaction was observed against Monkey IgA or Monkey IgG.|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|ELISA: 1:50,000 - 1:100,000
Western Blot: 1:1,000-1:5,000
Other Dilution: User Optimized
Western Blot of Peroxidase conjugated Goat anti-Monkey IgM mu antibody. Lane 1: Monkey IgM. Lane 2: none. Load: 100 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Observed size: 72 kDa for Rabbit IgG. Other band(s): none.
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