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Goat Anti-Monkey IgG (γ chain) [HRP],pAb

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Overview

Description	Goat Anti-Monkey IgG (γ chain) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Monkey IgG (gamma chain) peroxidase conjugated antibody generated in goat detects specifically Monkey IgG (gamma chain). This secondary peroxidase conjugated antibody anti-Monkey is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species	Goat
Antigen Species	Monkey
Conjugation	Peroxidase (Horseradish)
Immunogen	Anti-Monkey IgG (gamma chain) was produced by repeated immunization with monkey IgG gamma heavy chain in goat.

Properties

Subclass	IgG
Concentration	Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution	Restore with deionized water (or equivalent)
Specificity	MONKEY IgG gamma Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgG and Monkey Serum. No reaction was observed against Monkey IgA or Monkey IgM.
Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Applications

ELISA: 1:10,000 - 1:50,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized

Western Blot of Peroxidase conjugated Goat anti-MONKEY IgG gamma antibody. Lane 1: Monkey IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 kDa for Monkey IgG Gamma. Other band(s): none.