|Description||Goat Anti-Armenian Hamster IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. HRP Anti-Hamster IgG Secondary Antibody is designed for Western Blotting, ELISA and Immunohistochemistry. HRP conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development.|
|Antigen Species||Armenian Hamster|
|Immunogen||Armenian Hamster IgG whole molecule|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum. Greatly diminished reactivity will occur against Golden Syrian Hamster IgG.|
|Species Reactivity||Armenian Hamster|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|ELISA: 1:150,000 - 1:250,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized
ELISA results of purified Goat anti-Armenian Hamster IgG Antibody tested against purified Armenian Hamster IgG. Each well was coated in duplicate with 1.0 µg of Armenian Hamster IgG. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
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