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Catalog Products » Other Products » Goat Anti-Human κ chain,pAb

Goat Anti-Human κ chain,pAb

Description Goat Anti-Human κ chain antibody Unconjugated, pAb. Anti-Human IgG (kappa chain) antibody generated in goat detects specifically human IgG (kappa chain). This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Goat
Antigen Species Human
Conjugation Unconjugated
Immunogen Anti-Human IgG (kappa chain) was produced by repeated immunization with human IgG kappa light chain in goat.
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Subclass IgG F(ab')2
Concentration Liquid (sterile filtered) , 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross-reactivity against other human heavy or light chain isotypes.
Species Reactivity Human
Storage Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

ELISA: 1:25,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized




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Cat. No. A10247
Size
Price
1 mg $162.00
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