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Catalog Products » Other Products » Goat Anti-Human IgG F(c),pAb

Goat Anti-Human IgG F(c),pAb

Description Goat Anti-Human IgG F(c) antibody Unconjugated, pAb. F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Host Species Goat
Antigen Species Human
Conjugation Unconjugated
Immunogen Human IgG F(c) fragment

Subclass IgG F(ab')2
Concentration Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab) or Bovine, Horse, Mouse and Rat Serum Proteins.
Storage Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

ELISA: 1:1,500 - 1:7,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:500 - 1:5,000
Other Dilution: User Optimized

  • Goat Anti-Human IgG F(c),pAb
  • Goat Anti-Human IgG F(c),pAb

    Western Blot of Goat anti-F(ab')2 Human IgG F(c) Antibody Pre-AdsorbedLane 1: Human IgG F(c) Load: 100 ng per lanePrimary antibody: F(ab')2 Human IgG F(c) Antibody Pre-Adsorbed at 1:1000 o/n at 4°CSecondary antibody: DyLight™ 800 Donkey anti-goat at 1:20,000 for 30 min at RTBlock: Blocking Buffer for Fluorescent Western Blotting for 30 min at RTPredicted/Observed size: 28 kDa, 28 kDa




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Cat. No. A10249
Size
Price
1 mg $124.00
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