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Home » Gene Synthesis & Molecular Biology Services » Goat Anti-Human IgG F(c) [HRP],pAb

Goat Anti-Human IgG F(c) [HRP],pAb

*This product has been discontinued!*
Description Goat Anti-Human IgG F(c) antibody Peroxidase (Horseradish) conjugated, pAb. F(ab')2 Anti-Human F(c) antibody was generated in goat and detects specifically Human IgG F(c) fragment. This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Goat
Antigen Species Human
Conjugation Peroxidase (Horseradish)
Immunogen Human IgG F(c) fragment

Subclass IgG F(ab')2
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity F(ab')2 Anti-Human IgG Fc was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab) or Bovine, Horse, Mouse and Rat Serum Proteins.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:130,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized




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