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Home » Gene Synthesis & Molecular Biology Services » Goat Anti-Human IgG F(c) [BAC],pAb

Goat Anti-Human IgG F(c) [BAC],pAb

Description Goat Anti-Human IgG F(c) antibody Biotin conjugated, pAb. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Host Species Goat
Antigen Species Human
Conjugation Biotin
Immunogen Human IgG F(c) fragment

Subclass IgG F(ab')2
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab) or Bovine, Horse, Mouse and Rat Serum Proteins.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:125,000 - 1:175,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

  • Goat Anti-Human IgG F(c) [BAC],pAb
  • Goat Anti-Human IgG F(c) [BAC],pAb

    Western Blot of Goat anti-F(ab')2 Human IgG F(c) Antibody Biotin Conjugated Pre-AdsorbedLane 1: Human FcLoad: 100 ng per lanePrimary Antibody: F(ab')2 Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed at 1:1000 for 60 min RTSecondary antibody: HRP Conjugated Streptavidin at 1:40,000 for 30 min at RTBlock: Blocking Buffer for Fluorescent Western Blotting for 30 min at RTPredicted/Obsevered Size: 28 kDa/28 kDa

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Cat. No. A10256
1 mg $181.00