|Description||Goat Anti-Human IgG F(c) antibody R-Phycoerythrin (RPE) conjugated, pAb. F(ab')2 HUMAN IgG F(c) Pre-adsorbed Phycoerythrin Conjugated Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 HUMAN IgG F(c) Pre-adsorbed Phycoerythrin Conjugated Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.|
|Immunogen||Anti-Human IgG was produced by repeated immunization with Human IgG F(c) fragment in goat.|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab) or Bovine, Horse and Mouse Serum Proteins.|
|Storage||Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.|
Flow Cytometry: 1:100 - 1:250
IF Microscopy: 1:100 - 1:250
Other Dilution: User Optimized
Western blot of Phycoerythrin conjugated Goat F(ab’)2 Anti-Human IgG F(c) Pre-Adsorbed secondary antibody.Lane 1: Human Fc.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 28 kDa, 28 kDa for Human IgG F(c).Other band(s): None.
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