|Description||Rabbit Anti-Mouse IgG (H&L) antibody Unconjugated, pAb. F(ab')2 MOUSE IgG (H&L) Antibody Pre-Adsorbed was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 MOUSE IgG (H&L) Antibody Pre-Adsorbed is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.|
|Immunogen||Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.|
|Concentration||Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.|
|Storage||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|ELISA: 1:2,000 - 1:20,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:1,000 - 1:10,000
Other Dilution: User Optimized
SDS-PAGE of F(ab')2 Rabbit anti-Mouse IgG Antibody min x Human serum proteins. Lane 1: Reduced F(ab')2 Rabbit anti-Mouse IgG Antibody min x Human serum proteins. Lane 2: 5µL OPAL Pre-stained Marker MB-210-0500. Lane 3: Non-reduced F(ab')2 Rabbit anti-Mouse IgG Antibody min x Human serum proteins. Load: 1µg per lane. Predicted/Observed size: Non-reduced at 75 kDa , Reduced at 25 kDa.
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