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Goat Anti-Rabbit IgG F(ab')2,pAb

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Overview

Description	Goat Anti-Rabbit IgG F(ab')2 antibody Unconjugated, pAb. F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Host Species	Goat
Antigen Species	Rabbit
Conjugation	Unconjugated
Immunogen	Rabbit IgG F(ab')2 fragment

Properties

Subclass	IgG F(ab')2
Concentration	Liquid (sterile filtered) , 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab')2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Applications

ELISA: 1:10,000 - 1:50,000
Immunohistochemistry: 1:500 - 1:3,000
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized

Western Blot of Goat anti-F(ab')2 Rabbit IgG F(ab')2 Antibody Pre-AdsorbedLane 1: Rabbit IgG F(ab')2 Load: 100 ng per lanePrimary antibody: F(ab')2 Rabbit IgG F(ab')2 Antibody Pre-Adsorbed at 1:1000 o/n at 4°CSecondary antibody: DyLight™ 800 Donkey anti-goat at 1:20,000 for 30 min at RTBlock: Blocking Buffer for Fluorescent Western Blotting for 30 min at RTPredicted/Observed size: 28 kDa, 28 kDa Other band(s): antigen breakdown