• English
  • Sign In
  • Contact Us
×
Catalog Products » Other Products » Donkey Anti-Rabbit IgG (H&L) [RPE],pAb

Donkey Anti-Rabbit IgG (H&L) [RPE],pAb

Description Donkey Anti-Rabbit IgG (H&L) antibody R-Phycoerythrin (RPE) conjugated, pAb. F(ab')2 RABBIT IgG [H&L] Antibody Phycoerythrin conjugated Pre-adsorbed was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 RABBIT IgG [H&L] Antibody Phycoerythrin conjugated Pre-adsorbed is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Host Species Donkey
Antigen Species Rabbit
Conjugation R-Phycoerythrin (RPE)
Immunogen Anti-Rabbit IgG was produced by repeated immunization with Rabbit IgG whole moleculein goat.

Subclass IgG F(ab')2
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.
Species Reactivity Rabbit
Storage Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.


Flow Cytometry: 1:100 - 1:200
IF Microscopy: 1:100 - 1:200
Other Dilution: User Optimized

  • Donkey Anti-Rabbit IgG (H&L) [RPE],pAb
  • Donkey Anti-Rabbit IgG (H&L) [RPE],pAb

    Western blot of Phycoerythrin conjugated Donkey F(ab’)2 Anti-Rabbit IgG Pre-Adsorbed secondary antibody.Lane 1: Rabbit IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Phycoerythrin donkey secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG.Other band(s): None.


Cat. No.
Product Name
Price
A10193
Donkey Anti-Rabbit IgG (H&L) [ALP],pAb - 1 mg
$218.00
A10199
Donkey Anti-Rabbit IgG (H&L) [ALP],pAb - 1 mg
$153.00
A10195
Donkey Anti-Rabbit IgG (H&L) [BAC],pAb - 1 mg
$180.00
A10200
Donkey Anti-Rabbit IgG (H&L) [BAC],pAb - 2 mg
$114.00
A10190
Donkey Anti-Rabbit IgG (H&L) [FITC],pAb - 1 mg
$176.00
A10197
Donkey Anti-Rabbit IgG (H&L) [FITC],pAb - 2 mg
$105.00
A10192
Donkey Anti-Rabbit IgG (H&L) [HRP],pAb - 1 mg
$195.00
A10198
Donkey Anti-Rabbit IgG (H&L) [HRP],pAb - 2 mg
$129.00
A10196
Donkey Anti-Rabbit IgG (H&L) [TR],pAb - 1 mg
$176.00
A10201
Donkey Anti-Rabbit IgG (H&L) [TR],pAb - 2 mg
$114.00
A10186
Donkey Anti-Rabbit IgG (H&L) [TRITC],pAb - 2 mg
$105.00
A10188
Donkey Anti-Rabbit IgG (H&L),pAb - 1 mg
$123.00
A10194
Donkey Anti-Rabbit IgG F(c) [BAC],pAb - 2 mg
$149.00
A10189
Donkey Anti-Rabbit IgG F(c) [FITC],pAb - 2 mg
$124.00
A10191
Donkey Anti-Rabbit IgG F(c) [HRP],pAb - 2 mg
$168.00
A10187
Donkey Anti-Rabbit IgG F(c),pAb - 2 mg
$114.00
 


Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. A10276
Size
Price
500 µg $264.00