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Goat Anti-Rat IgG F(c) [HRP],pAb

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Overview

Description	Goat Anti-Rat IgG F(c) antibody Peroxidase (Horseradish) conjugated, pAb.
Host Species	Goat
Antigen Species	Rat
Conjugation	Peroxidase (Horseradish)
Immunogen	Rat IgG F(c) fragment

Properties

Subclass	IgG F(ab')2
Concentration	Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution	Restore with deionized water (or equivalent)
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse, Human Serum Proteins.
Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Applications

ELISA: 1:50,000 - 1:100,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:10,000
Other Dilution: User Optimized

ELISA results of purified F(ab')2 Goat anti-Rat IgG F(c) antibody Peroxidase Conjugated Min x Bv, Hs, & Hu serm proteins tested against purified Rat IgG F(c). Each well was coated in duplicate with 1.0 µg of Rat IgG F(c). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.