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Donkey Anti-Goat IgG (H&L) [HRP],pAb

Description Donkey Anti-Goat IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Goat IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically goat IgG. This secondary peroxidase conjugated antibody anti-Goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Donkey
Antigen Species Goat
Conjugation Peroxidase (Horseradish)
Immunogen Anti-Goat IgG (H&L) was produced by repeated immunization with goat whole molecule in donkey.
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Subclass IgG F(ab')2
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity Fab Goat IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
Species Reactivity Goat
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:200,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

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Cat. No. A10279
1 mg $187.00