Catalog Products » Other Products » THETM 25-OH Vitamin D3 Antibody (32F9C4), mAb, Mouse

25-OH VD3 (32F9C4), mAb, Mouse

Synonyms THETM 25-OH Vitamin D3 Antibody (32F9C4), mAb, Mouse
Description Vitamin D plays an important role in calcium homeostasis and metabolism. Vitamin D3 and vitamin D2 are two forms of vitamin D in human body, both of which only a few foods contain. The deficiency of vitamin D in human body can cause a threat of diseases such as osteomalacia, rickets and diabetes. 25-OH Vitamin D2 and 25-OH Vitamin D3 as vitamin D metabolites are measured in human serum to evaluate vitamin D level. Vitamin D supplements are considered as an effective way to treat or to prevent the diseases.
Antigen Species Human
Conjugation unconjugated
Purification Protein A/G affinity column

Production Cultured in vitro under conditions free from animal-derived components
Clone ID 32F9C4
Subclass IgG2a
Concentration >4mg/ml
Specificity Mab 32F9C4 recognizes 25-OH Vitamin D3 and has no cross-reactivity with 25-OH Vitamin D2. The specificity of the antibody is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding. The cross-reactivity data obtained in competitive ELISA system is as follows:
Compound Cross-reactivity (%)
25-OH VD3 100
1α, 25-OH VD3 78
24R, 25-2OH VD3 36
25-OH VD2 0.058
1α, 25-(OH)2 VD2 0.094
VD2 0.004
VD3 0.026
Formulation PBS, pH 7.4, containing 0.03% Proclin 300
Temperature, Time Result
-80 °C, 21 days OK
-20 °C, 21 days OK
4 °C, 21 days OK
20°C, 21 days OK
37°C, 21 days OK
40°C, 21 days OK
Storage For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Test applications: ELISA, CLIA, GICA, LF

  • 25-OH VD3 (32F9C4), MAb, Mouse
  • 25-OH VD3 (32F9C4), MAb, Mouse

    Tested at 37℃、20℃、4℃、-20℃ separately for 21 days, stability is high, CV<10%

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Cat. No. V00101
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