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Mn2+ quench method

A method for observation of the control of bivalent cation flux across the plasma membrane of a cell. Mn2+ is used in place of Ca2+ because, unlike Ca2+, it does not accumulate in intracellular sites. The entry of Mn2+ into a cell is monitored by the quenching of fluorescence of a fluorophore (fura-2) with which the cell has been loaded. Jacob, R. (1990) J. Physiol. (London) 421, 55-77

Recommended reading: next generation sequencing

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