A method for cleavage of double-stranded DNA at specific restriction sites. A suitable restriction site is selected, around which the base sequence is known, and an oligonucleotide, up to 50 or 60 bases in length, is synthesized to complement it. It is hybridized to the DNA to form a triple helix, which is stabilized with the help of the RecA protein. Methylation then blocks all but the protected restriction site, so that subsequent treatment with the restriction nuclease, in the absence of the protecting oligonucleotide and RecA protein, will cleave only at the designated site. The use of two protecting oligonucleotides for sites that span a sequence of interest allows excision of a specific fragment from a DNA preparation as complex as the human genome. Ferrin, L.J. and Camerini-Otero, R.D. (1991) Science 254, 1494-1497
GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.
GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.
This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.
GenScript recombinant protein and rAb services provide high quality recombinant proteins and rAbs for a variety of downstream research applications.
One-stop service from Sequence to Protein starting from $1600, 4 weeks.
GenScript's BacuVance baculovirus expression system was developed by our in-house team of scientists for virus production and expression of recombinant proteins from baculovirus-infected insect cells.
Mammalian Transient Expression
Proprietary High Density (HD) expression system, enhance the protein yield up to 100 fold, achieve antibody titers up to 3 g/L.
Reagents for COVID-19 Research
Gram level, ready to ship RBD proteins, ACE2 assay cell lines and pseudovirus.
If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]