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Easson and Stedman plot

A graphical method for determination of enzyme-inhibitor dissociation constants for non-competitive inhibitors, especially those that bind very tightly. Where I is the total inhibitor concentration, E is the total enzyme concentration and is the ratio of inhibited to non-inhibited rates under the same conditions, I/ is plotted on the ordinate (y-axis) against 1/(I-) on the abscissa (x-axis) to give a straight line that intersects the ordinate at E; its slope is Kd, the dissociation constant. (see also Cornish-Bowden plot; Dixon plot; Hunter and Downs plot)

Learn more about restriction enzymes.

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If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]

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