Click Peptide Service
Hydrophobic peptides are difficult to prepare and handle, making their study or use in biological research difficult. For example, β-amyloids, which are highly hydrophobic peptides, are prone to natural self-assembly. These peptides aggregate and deposit on organs, forming debilitating plaques that are involved in the progression of many neurodegenerative diseases such as Alzheimer's and Parkinson's, as well as other diseases such as diabetes and Huntington's. β-amyloids are targets for developing effective therapies to treat these diseases, but these studies are often hindered by uncontrollable aggregative nature of β-amyloids.
Key Features1. Click Peptide Synthesis allows control over
Our Click Peptides are synthesized using the O-acyl isopeptide method, which was developed by Taniguchi, et al (Figure 1). We use a special synthesis method to incorporate a strategically placed o-acyl bond in place of a naturally occurring n-acyl bond in the native peptide. The resulting peptide, called an o-acyl isopeptide, exhibits enhanced solubility and minimal peptide self-assembly, due to a reduction in intramolecular hydrogen bond interactions between peptide chains.
Figure 1: Conversion of Click Peptide to native peptide via pH change
ReferenceAtsuhiko Taniguchi, Youhei Sohma, et al. Click peptide: Chemical Biology-oriented analogues of Alzheimer's amyloid β peptide 1–42. J. Peptide Science. Nov 2006; 12(12): 823-828
26-O-acyl β-amyloid (1-42) click peptide was successfully synthesized, the β-ester bond in which can be quickly and quantitatively converted to a native Gly25-Ser26 amide bond via a pH-dependent O-N intramolecular acyl migration reaction (t1/2 =1 min, pH 7.4, 37℃) at a hydroxyamino acid residue. Namely, upon this pH-triggered conversion (pH-click), the non-aggregative and water-soluble precursor (click peptide) can produce the monomer with a random-coil structure under physiological conditions (pH 7.4, 37℃). The structure information is as follows.
Scheme 2: β-amyloid (1-42) click peptide
The following are HPLC reports of (Scheme 3) purified β-amyloid (1-42) native peptide converted from β-amyloid (1-42) click peptide (precursor) and (Scheme 4) purified 26-O-acyl β-amyloid (1-42) click peptide (precursor).
(Scheme 3) HPLC report of purified β-amyloid (1-42) native peptide converted from β-amyloid (1-42) click peptide (precursor)
(Scheme 4) HPLC report of purified 26-O-acyl β-amyloid (1-42) click peptide (precursor)
Quotations and Ordering
- For quotations, please use our Secure Instant Online Quotation/Order system. However, you may also contact us by email, phone (1-732-885-9188), fax (1-732-210-0262), or via our Secure Messaging System.
- Order can be placed by email, phone, or fax with either a PO (Purchase Order) or credit card. Please submit your peptide sequence at email@example.com using our Click Peptide Order Form.
- We accept POs and major credit cards (). A 7% New Jersey sales tax will be applied to orders shipped to New Jersey. Your credit card will be billed under "GenScript" Click here to download our credit reference form. For international orders, we must apply the full charge at the time the order is placed. In the unlikely event that any given order cannot be filled, our guarantee will take the form of a full refund.
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