Resources » Technical Resource Centers » Gene Technical Resources » Molecular Cloning Center » DNA Ligation Troubleshooting
Component | Final concentration/amount |
---|---|
water |
to 10 µL |
Ligase buffer (with ATP) |
1X |
Vector |
25 ng |
Insert |
75 ng |
T4 DNA ligase |
0.1 to 1 Weiss unit |