Resources » Technical Resource Centers » Gene Technical Resources » Molecular Cloning Center » DNA Transfection Troubleshooting
Transfection protocols vary based on cell type to be transfected, transfection method, and transfection reagent. The common causes of transfection failure are decrease transfection efficiency and decreased cell viability. Use the table below to troubleshoot transfection experimental failure. Ensure the quality of your target construct by getting the desired gene in the vector you want the easy way with GenEZ™ ORF Clones. Start with a Search for your gene.
Problem | Cause | Solution |
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Low transfection efficiency |
DNA concentration too low or DNA is degraded |
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Complexes not properly formed |
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Culture medium contains contaminants |
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Cells have suffered mechanical damaged |
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Transfection reagent has been compromised |
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Low cell viability after transfection |
DNA used for transfection was contaminated |
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Target cells are compromised |
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Cell density too low |
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