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eBlot® L1 Fast Wet Transfer System
9-17 minutes transfer system with ultra-efficiency and simplicity
- Excellent transfer efficiency: Better transfer efficiency than traditional wet transfer
- Simple: Easy to assemble, one button operation and customizable programs available
- Comprehensive: Outstanding performance for small, medium and large proteins
- Fast: Complete transfer in 9-17minutes.
- Efficient: Simultaneously transfer two mini gels
- Compatible: Highly compatible with different types of precast or homemade gels
eBlot® L1 is a highly efficient wet protein transfer system, which uses a patented technology developed by GenScript. eBlot® L1 combines the high stability and efficiency of the traditional wet transfer with the rapidness and convenience of the semi-dry transfer system. The device allows the efficient transfer of small, medium and large molecular weight proteins within 9-17 minutes.
eBlot® L1 allows fast and efficient transfer of 1 or 2 mini gels at a time. The system comes with conveniently designed transfer stacks/consumables (sold separately). The setup takes less than 2 minutes and can be performed just with the dry sponge.
Embrace the brand new experience that eBlot® L1 brings you!
- Even better transfer efficiency compared to traditional wet transfer
- Highly efficient transfer of both low and high molecular weight proteins within 9-17 minutes
- Super easy to assemble, with one button operation and customizable programs
Make dry assembly possible, no messy dripping sponges anymore.
Customizable programs make it possible to satisfy any of your special needs.
|Wet transfer||Transfer Device A||Transfer device B||eBlot L1|
|eBlot L1 Transfer sandwich without membrane (with buffers for NC) - 1 kit||
|Complete eBlot L1 Transfer sandwich (NC) - 1 kit||
|eBlot L1 Transfer sandwich without membrane (with buffers for PVDF) - 1 kit||
|Complete eBlot L1 Transfer sandwich (PVDF) - 1 kit||
|Small Two Pass Screw Cap ( Fit 5L and 10L container)||-||L00741|
|WB Reaction Box||-||L00745|
eBlot L1 Fast Wet Protein Transfer System Quick Guide
Note: For complete instructions and advanced programming, please refer to the manual.
|Step 1. After gel electrophoresis, dissemble the gel cassette and put the gel in a tray with about 100 ml of water for 1 minute. For Nitrocellulose (NC) membrane, add enough NC Equilibration buffer into a clean plastic container and soak the membrane for about 1 minute. For PVDF membrane, wet the membrane with ethanol or methanol first, then soak the membrane in PVDF Equilibration buffer for about 1 minute.|
|Step 2. Open the gel transfer cassette and lay it flat on the table. Locate the larger metal mesh (the side marked with +) as illustrated below:|
|Step 3. On the larger metal mesh, assemble the transfer sandwich in the following order: Sponge, equilibrated membrane, gel, sponge.
|Step 4. Close the transfer cassette, pick a channel (A or B) and insert the assembled cassette into the channel.
Note: Please insert the cassette with the side marked "Front" facing you.
|Step 5. For Channel A, push "Start A" button, it will start flashing and the timer on the screen will begin the countdown. Machine will beep when the countdown reaches 0, push "Start A" again to finish the process. For Channel B, push "Start B" and follow similar procedure as above.|
|Step 6. Take out the transfer cassette and dissemble the transfer sandwich. Discard the gel and sponges. Rinse the membrane in water and proceed to the next process.
Note: please rinse the transfer cassette with water and let it dry after use.