GenScript’s DNA library preparation technology is fully compatible with Illumina sequencer. We offer mechanical and enzymatic fragmentation for various sample types, from genomic DNA to low input difficult samples, suitable for either manual prep or automatable workflow. Our library preparation kits are also fully compatible with our target enrichment hybridization kits and panels.
- High quality output to maximize the coverage and complexity to meet your sequencing project needs
- Optimal fragment size to efficiently utilize your sequencer and ease your data analysis
- From small scale manual prep to high-throughput automated library prep with enzymatic fragmentation
- Cost-efficient but superior quality
GenTrack And GenNature Library Prep Kit Flyer
Economic DNA Library prep, Mechanical fragmentation, simple workflow of 2.5 hr
GenTrack DNA Library Preparation Kit is designed for genomic DNA samples that have been mechanically fragmentized. This kit offers all enzymes and reagents for end-repair, A-tailing, adapter ligation and amplification, as well as high quality purification beads.
The GenTrack DNA Library Prep Kit offers:
- Enhanced sequencing efficiency: Uses high recovery magnetic beads to achieve optimal size distribution
- Simple workflow without compromising the quality:
- Master-mixed reagents to reduce reagent containers and pipetting
- Gel-free size-selection for fragmented DNA with optimized beads conditions
- High throughput—96 dual indexes adaptor pairs compatible with all Illumina sequencing platform
Workflow-Easy 4 Steps!
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Check the Fragmented DNA
This step will check whether the size distribution of fragmented DNA is expected.
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End-Repair and A-Tailing
This step converts the overhangs resulting from fragmentation into blunt ends, repair the nick and then adding an "A" nucleotides to the 3’ end of the blunt fragments.
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Adapter Ligation
This step adds the adapters with index to the ends of the DNA fragment after end preparation.
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Amplification
This PCR step selectively enriches the DNA fragments with adapter on both ends, and amplifies the amount of DNA libraries output for sequencing.
GenTrack DNA Library Preparation Kit Performance and specifications: Read More »
Verified by multiple applications, GenTrack DNA Library Preparation Kit not only exceeds the input quantity requirements of various Illumina sequencing platforms, but also shows nice size distributions to enhance sequencing efficiency.
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Enough output quantity meets the requirements of various sequencing machine.
2100 Bioanalyzer detection verifies that fragments of different sizes meet the requirements of the sequencing machine.
Sample input types: genomic DNA, virus plasmid, virus genome (RNA->DNA is needed before library construction), plasmid, PCR fragment
Sample input amounts: 1-1000 ng DNA (>10 ng gDNA,
<10 ng PCR fragment)
Library preparation time: 2.5 hrs
Compatible adaptors (sell separately): GenTrack 96 dual-index adaptors, custom adaptors
System compatibility: Illumina Platform
Tech document: Read More »
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GenTrack DNA Library Preparation Kit contains a double-ended unique tag adapter and a finished reagent for each step, providing you with a one-stop solution for the library, followed by a hybrid capture probe kit a double-ended, unique labeled adapter with a very low cross-contamination rate, making it an excellent choice for accurate sequencing data.
Free sample
Mechanical fragmentation or fragmentase, low input DNA, cfDNA –Recruiting testers
GenTrack Library Preparation kit V2.0 enables fragmentase fragmentation besides mechanical shearing, making the kit more flexible for both manual or automated library preparation.
- Flexible DNA fragmentation approach: Mechanical or fragmentase
- Less DNA loss during fragmentation, perfect for low-input DNA, cfDNA
- Optimal recipe to improve ligation efficiency and reduce adaptor dimer, expecially for impure PCR fragment library prep to reduce sample mis-assignment
GenTrack DNA Library Preparation Kit V2.0 Performance and specifications: Read More »
GenTrack DNA library preparation kit V2.0 has much higher output, lower duplication rate and lower soft clip ratio compare to industrial leading competitor’s DNA library preparation kit.
Order:
Please contact [email protected]. Our technical account managers will contact you in 24 hours.
Tagmentation, automatable, simplest workflow of 1.5 hr library prep
GenNature DNA Library Preparation Kit is designed for genome DNA samples that have been enzymatic fragmentized. The kit offers all enzymes and reagents for fragmentation and amplification in only two steps.
- Optimized and complete protocol in less than 90 min
- Simple workflow and automatable-
- Single enzymatic reaction for both fragments and adapter addition
- No mechanical fragmentation/shearing or other tedious steps required
- Minimum 25 ng input of DNA needed
- High throughput— up to 384 dual index pairs available, compatible with all Illumina sequencing systems
GenNature Library Preparation Kit – Easy 2 Steps!
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Tagment DNA
This step will fragment DNA and tag the fragmented DNA with adapter at the same time, with transposase.
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PCR Enrichment
This step amplifies tagmented DNA and adds index adapters, including Index 1 (GN) and Index 2 (GS) sequence for barcoding and common sequence (P5 and P7) for sequencing.
GenNature DNA Library Preparation Kit Performance and specifications: Read More »
25 ng input DNA (1ul TAT, 12 PCR cycles, yield: 800 ng)
Sample input types: genomic DNA, plasmid, cDNA, virus genome (RNA->DNA is needed before library construction), plasmid, PCR fragment
Sample input amounts: >25 ng gDNA
Library preparation time: 1.5 hrs
Compatible adaptors (sell separately): GenNature 4x 96 dual-index adaptors, custom adaptors
Suitable for: Whole Genome Sequencing, Whole Exome Sequencing, Target Sequencing
System compatibility: Illumina Platform
Tech document: Read More »
Order:
Tagmentation, automatable, simplest workflow of 1.5 hr library prep, low input DNA -- Recruiting testers
GenNature DNA Library Preparation Kit is designed for DNA samples by tagmentation. The simple two step approach and optimized PCR enrichment protocol allows you to prep your low input DNA library via automation.
- Optimized and complete protocol in less than 90 min
- Simple workflow and automatable-
- Single enzymatic reaction for both fragments and adapter addition
- No mechanical fragmentation/shearing or other tedious steps required
- Low DNA input—Only 500 pg input of DNA needed
- High throughput— up to 384 dual index pairs available, compatible with all Illumina sequencing systems
GenNature Library Preparation Kit V.20 – Easy 2 Steps!
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Tagment DNA
This step will fragment DNA and tag the fragmented DNA with adapter at the same time, with transposase.
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PCR Enrichment
This step amplifies tagmented DNA and adds index adapters, including Index 1 (GN) and Index 2 (GS) sequence for barcoding and common sequence (P5 and P7) for sequencing.
GenNature DNA Library Preparation Kit Performance and specifications: Read More »
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100 ng input DNA (5ul TAT, 9 PCR cycles, yield: 1 μg)
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50 ng input DNA (3ul TAT, 10 PCR cycles, yield: 1 μg)
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25 ng input DNA (1ul TAT, 12 PCR cycles, yield: 800 ng)
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1 ng input DNA (1ul TAT diluted 20-fold, 17 PCR cycles, yield: 1 μg)
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500 pg input DNA (1ul TAT diluted 25-fold, 16 PCR cycles, yield: 400 ng)
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200 pg input DNA (1ul TAT diluted 40-fold, 20 PCR cycles, yield: 1 μg)
Sample input types: genomic DNA, virus plasmid, virus genome (RNA->DNA is needed before library construction), plasmid, PCR fragment
Sample input amounts: >500pg to 1000ng
Library preparation time: 1.5 hrs
Compatible adaptors (sell separately): GenNature 4x 96 dual-index adaptors, custom adaptors
Suitable for: Whole Genome Sequencing, Whole Exome Sequencing, and Custom Targeted Sequencing, Amplicon Sequencing
System compatibility: Illumina Platform
Order:
Please contact [email protected]. Our technical account managers will contact you in 24 hours.
